Helicobacter pullorum in Chickens, Belgium

نویسندگان

  • Liesbeth M. Ceelen
  • Annemie Decostere
  • Kathleen Van den Bulck
  • Stephen L.W. On
  • Margo Baele
  • Richard Ducatelle
  • Freddy Haesebrouck
چکیده

A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and identification of Helicobacter pullorum from caecal content of broiler chickens in Mashhad, Iran

The presence of Helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. In this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in Mashhad suburb were tested for the presence of H. pullorum using modified conventional culture method by combination of culturing on Brucella sheep blood agar and a filte...

متن کامل

Investigation of Helicobacter pullorum Occurence in Chicken in the Marmara Region of Turkey

The present study was to investigate the incidence of Helicobacter pullorum in caecum and colon of 96 broiler chickens. All samples were obtained from different commercial broiler slaughtering facilities in Marmara region of Turkey. The presence of H. pullorum was assessed by conventional culture method and specific polymerase chain reaction (PCR) test. H. pullorum were not isolated from any of...

متن کامل

Prevalence of Helicobacter pullorum in broiler chickens reared in intensive and extensive farms

Helicobacter pullorum infections are underestimated due to phenotypic similarities between the genera Helicobacter and Campylobacter. The aim of this work was to estimate the Helicobacter pullorum prevalence in 209 caecum contents collected from Italian broilers reared in 33 intensive and 9 extensive farms by using a Steele-McDermott membrane filter method modified. The isolate identifications ...

متن کامل

Genome dynamics and molecular infection epidemiology of multi-drug resistant Helicobacter pullorum isolates obtained from broiler and country chickens in India

Genome dynamics and molecular infection epidemiology of multi-drug resistant 1 Helicobacter pullorum isolates obtained from broiler and country chickens in India 2 3 Shamsul Qumar, Mohammad Majid, Narender Kumar, Sumeet K. Tiwari, Torsten 4 Semmler, Savita Devi, Ramani Baddam , Arif Hussain, Sabiha Shaik, Niyaz Ahmed* 5 6 1. Pathogen biology laboratory, Department of Biotechnology and Bioinform...

متن کامل

Prevalence of Helicobacter pullorum in conventional, organic, and free-range broilers and typing of isolates.

Helicobacter pullorum represents a potential food-borne pathogen, and avian species appear to be a relevant reservoir of this organism. In this study, the prevalence of H. pullorum was investigated at 30 conventional farms where 169 ceca from 34 flocks were tested, at eight organic farms where 39 ceca from eight flocks were tested, and at seven free-range farms where 40 ceca from eight flocks w...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 12  شماره 

صفحات  -

تاریخ انتشار 2006